Národní úložiště šedé literatury Nalezeno 2 záznamů.  Hledání trvalo 0.01 vteřin. 
Enzymatic peptide synthesis - simulation of nucleophile excess
Klein, Jens Uwe ; Čeřovský, Václav
Gradual addition of the acyl component in small doses to a solution of the nucleophile and enzyme resulted in the yield improvement of protease-catalyzed peptide synthesis.
Desoctapeptideinsulin: Substrate of ŕ-chymotrypsin
Koubová, V. ; Barthová, J. ; Barth, Tomislav ; Bezouška, K. ; Ubik, Karel ; Kašička, Václav
Desoctapeptideinsulin (DOI) contains three tyrosine residues and therefore it represents a suitable substrate for ŕ-chymotrypsin. The detailed enzymic study reveated a series of intermediates and final products. The bonds formed by tyrosine A14 and B16 are attacked as the first, and the products are further desintagrated.

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